Comparison of Body Weight and the Weight of Stomach after Simultaneous Administration of Ibuprofen and L-Arginine Albino Rats

Abstract

Objective: To observe the effects of ibuprofen and L-Arginine when given simultaneously on the body weight and absolute weight of stomach of albino rats and statistical analysis of the results.

Study Design: A prospective experimental study.

Place and Duration of study: This study was conducted at Department of Anatomy, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre Karachi during 2008.

Materials and Methods: This study was conducted in the Department of Anatomy, Basic Medical Sciences Institute Jinnah Postgraduate Medical Center Karachi where 45 albino rats of either sex between 90-120 days were selected and were divided into three groups, ‘A’, ‘B’ and ‘C’, containing 15 animals each, which were further sub-divided into three sub-groups containing 5 animals each according to time of sacrifice, i.e. 4, 6, and 8 weeks respectively.  Group ‘A’ served as control. Group ‘B’ received ibuprofen at the dose of 70 mg per kilogram body weight per day with feed and L-Arginine 300 mg per kilogram body weight per day with feed. Animals were weighed on sartorius balance before and after their stipulated time period. Animals were fixed on a dissecting board, the abdomen was opened with a long midline incision and the stomach was removed and opened along the greater curvature with an incision extending from cardiac to the pyloric end. After removing the contents of the stomach the absolute weight of the stomach was recorded on a Sartorius balance.

Results: Observations on body weight (G) in Group-‘A’ There was a significant increase (P<0.05) in the weight of all subgroups. In Group-‘B’ their was moderate decrease in weight subgroup ‘B’1 and significant decrease in subgroups ‘B’2 B’’3 when compared to control. No difference was observed in Group C when compared with control. Observations of absolute weight of stomach (G) the weight was increased significantly (P<0.05) when compared with control. No difference in weight was noted between Group ‘C’ and control group.

Conclusion: The effects of ibuprofen on the body weight and absolute weight of the stomach could be minimized when given simultaneously with L-Arginine.