Fabrication & Analysis of the Nanohydroxyapatite / Chitosan Membranes for Rejuvenation of Periodontal Tissues

Abstract

Objective: To fabricate Nanohydroxyapatite/Chitosan barrier membranes loaded with Amoxicillin nanoparticles and evaluate their structural and antibacterial properties for periodontal tissue regeneration

Study Design: It is a cross sectional study.

Place and Duration of Study: This study was conducted at the RIPS (Riphah Institute of Pharmaceutical Sciences) Islamabad in collaboration with IST (Institute of Space and Technology) Islamabad April 2020 to February 2021 for a duration of ten months.

Materials and Methods: The membranes of nanohydroxyapatite/chitosan incorporated with the drug loaded nanoparticles were fabricated by solvent casting method followed by lyophilization. The membranes were characterized using SEM. Antibacterial activity of the membranes was observed using disc diffusion method and the zone of inhibition was measured against Staphylococcus aureus and Escherichia coli

Results: The membranes exhibited a porous structure with all the components blended well. The zone of inhibition measured against Staphylococcus aureus was 24.6±0.4 mm and that against Escherichia coli measured 22.3±0.4mm.

Conclusion: It can be suggested from the results that the fabricated membranes incorporated with drug loaded nanoparticles show their potential to be used in the periodontal regenerative therapy possessing adequate structural and antibacterial features.