Protective Effect of Tinospora Bakis (Miers) Methanolic Extract on Gentamicin-Induced Nephrotoxicity in Rats

Abstract

Objective: To investigate the potential nephroprotective effects of (Tinospora Bakis) methanol extract against gentamicin-induced kidney damage in rats.

Study Design: Experimental study

Place and Duration of Study: This study was conducted at the Central Veterinary Research Laboratory, Khartoum, Sudan from 1st February 2022 to 31st January 2023.

Methods: Wister albino rats were used as control groups (gentamicin only) and treatment groups (gentamicin + Tinospora Bakis extract at different doses). Induction of nephrotoxicity in rats using gentamicin. Administration of Tinospora Bakis methanol extract at different doses. Measurement of serum urea, creatinine, total proteins, and electrolytes. Histopathological analysis of kidney tissues.

Results: Gentamicin increased serum markers of kidney damage (urea, creatinine, total proteins).

Tinospora Bakis extract reduced these markers, indicating a protective effect. Gentamicin decreased serum electrolytes (sodium, potassium), which were restored by Tinospora Bakis extract. Histopathological analysis Tinospora Bakis extract prevented Gentamicin-induced nephrotoxicity, supported the protective effect of the extract.

Conclusion: Tinospora Bakis methanol extract exhibits nephroprotective activity against gentamicin-induced kidney damage, possibly due to the presence of bioactive compounds. Further research is needed to identify and characterize these compounds.