Effects of Nicotine Treatment on Viability of Inflamed Reconstituted Model of Oral Mucosa

Abstract

Objective: The objective of this assignment is to evaluate the effects of nicotine for over a span of 5 minutes and more than 24 hours on an inflamed stratified epithelial layer.

Study Design: Observational research

Place and Duration of Study: This study was conducted at the Department of Oral Pathology at Bart's and the London Queen Mary School of Medicine and Dentistry at Queen Mary, University of London from July 2018 to July 2019 for a period of one year.

Materials and Methods: The effects of nicotine on an in vitro Viability of Inflamed Reconstituted Model of Oral Mucosa are the subject of this study. Skin Ethic Laboratories, Nice, France, produced and supplied the reconstituted human epithelial model used in this study. The MTT test was used to determine the effect of different nicotine treatments on tissue viability.

Results: The effect of nicotine on the viability of inflamed stratified epithelium layer viability after 5 minutes and 24 hours with working solutions (10M, 100M, 1mM, and 10mM) on inflamed oral mucosa was determined to be insignificant.

Conclusion: This study found that all nicotine concentrations applied after 5 minutes and 24 hours had no effect on the viability of inflamed tissue.