Protective Effect of Withania Somnifera Root Extract Against Cisplatin Induced Nephrotoxicity Through Renal Function Analysis in Albino Wistar Rats

Abstract

Objective: To observe the nephroprotective effects of W. Somnifera root extract against cisplatin induced 
nephrotoxicity through biochemical parameters in albino Wistar rats. 
Study Design: Experimental study 
Place and Duration of Study: This study was conducted at the Baqai Medical University, Karachi fromNovember 
2018 till February 2019. 
Materials and Methods: For this study 80 adult male Albino Wistar rats were divided in to four groups, 20 rats in 
each group. Group A served as control group,group B received inj. Cisplatin(1mg/kg intraperitoneally) for 7 days, 
group C received W. Somnifera root extract (500mg/kg) for 15 days before cisplatin treatment and thereafter 
concurrently with cisplatin for last 7 days. Whereas group D was given only W. Somniferaroot extract (500mg/kg) 
for 22 days. 1stblood samples of all the groups were collected at the start of the study. Second blood samples of 
group A, C and D were taken on 23rd day and of group B was taken on day 8th of experiment. Estimation of serum 
urea and creatinine levels were done by using standard Laboratory kits. 
Results: In this study mean (+ SD)serum urea level of group A, B, C & D were 31.853+ 8.3258 mg/dl, 251.495 + 
95.4603 mg/dl, 35.570+ 22.1801 mg/dl and 30.700+ 6.2149mg/dl respectively. While mean (+ SD) serum creatinine 
level of group A, B, C & D were 0.538+ 0.0656 mg/dl, 2.109+ 0.9247 mg/dl, 0.606+ 0.1911 mg/dl and 0.521+ 
0.0495mg/dl respectively. Significant (p<0.05) mean serum urea and creatinine level differences were observed 
when WS pretreated and cisplatin treated Group C was compared with cisplatin group B indicative of correction of 
raised serum urea and creatinine level. While comparison with group A & D showed insignificant changes. 
Conclusion: It is concluded from our study that Cisplatin induced renal toxicity in albino Wistar rats may be 
corrected by W. Somnifera root extract through the renal function analysis (serum urea and creatinine levels).