A Light Micrscopic Study of Steatosis in Non Alcoholic Fatty Liver Disease and its Correlation with Fibrosis

Abstract

Objective: To assess the steatosis by microscopic study of liver biopsies in patients labeled as fatty liver on ultrasound and study its association with fibrosis.

Study Design: Case series study.

Place and Duration of Study: This study was conducted at the Gastroenterology unit of Hayatabad Medical Complex and Histology laboratory of Khyber Medical College and Histopathology Lab of CMH, Peshawar from June 2016 to December 2016.

Materials and Methods: Ten cases diagnosed as fatty liver on ultrasound were selected for liver biopsy followed by histological study. They were divided in to two groups. Group 1: between the ages of 45-65 years, obese having diabetes and hyperlipidemia. Group 2: 25-44 years, overweight, diabetic and hyperlipidemic.

Results: Histological examination clearly indicates that 60% of total cases developed grade 1 steatosis, while 30% and 10% of cases revealed grade 2 and 3 respectively. The results revealed highly significant association between the two groups and that of steatosis grade after application of Chi square. Azonal/panacinar steatosis was found in 60% of total cases while 10% and 30% of cases were reported with zone 1 and 3 steatosis. Only single case did not develop fibrosis although, the rest of patients revealed various stages of fibrosis. Stage 4 (cirrhosis) could not be recorded in any of the case.

Conclusion: Mix pattern of steatosis is present, with higher incidence of steatosis in old and obese patients having diabetes and hyperlipidemia. Furthermore, there exists no substantial correlation between steatosis grade and fibrosis stage.