Investigating Effects of Curcuma Longa on the Liver Enzymes and Histopathological Examination in Chemical Induced Liver Injury in Rats

Abstract

Objective: The present research investigated the hepatoprotective and histoprotective potential of Curcuma longa (CL) against chemical induced liver injury in laboratory male Wistar rats.

Study Design:  Experimental study

Place and Duration of Study: This study was conducted at the Animal House, Al-Tibri Medical College and Sindh Agriculture University from September 2016 to January 2017.

Materials and Methods:  60 adult male rats (Wistar strain), of 150- 250 grams, were randomly divided into 3 groups. Group A. Control (n=20) Rats were given isotonic saline (0.9%) orally daily for 4 weeks, Group B. (n=20) Rats were given Carbon tetrachloride (CCl₄) orally on alternate day for 4 weeks; Group C. (n=20) Rats were fed received Carbon tetrachloride (CCl₄) + Curcuma longa (250 mg/kg) orally on alternate days for 4 weeks. Blood samples were collected by cardiac puncture. Liver tissue 3-5μ thick sections were stained with H & E stain and examined by light microscopy. Statistical analysis was performed on Statistix 9.0 (USA) (P-value of ≤ 0.5)

Results: The present study shows hepatoprotective and histoprotective potential of Curcuma longa (CL) against chemical (carbon tetrachloride) induced liver injury. Curcuma longa improved Liver enzymes, serum superoxide dismutase, glutathione peroxidase, catalase and liver histology (P <0.05).

Conclusion: Curcuma longa exerts hepatoprotective and histoprotective effects, and mitigates oxidative damage induced by carbon tetrachloride.