Ameliorative Role of Jamul (Syzygiumcumini) against Chromium Induced Histopathological Reproductive Anomalies

Abstract

Objectives: Heavy metals are global environmental pollutants and one of them chromium (Cr); frequently used in Pakistani traditional sweets without medical authentications; may induce reproductive anomalies.

Study Design: Observational Study.

Place and Duration of Study: That study was conducted at the Medicine Deptt Sh. Zayed Hospital, Lahore and Department of Biology, Punjab University Lahore from Oct-2016 to Dec-2016.

Materials and Methods: A randomized study was conducted on 30 male mice (Musmusculus), maintained for 15days as,G-1 (C) control, G-2 (Cr)K₂Cr₂O₇ (50ppm for 10days ad-libitum)andG-3 (Cr-J) as G-2 but received Jamul (Syzygiumcumini) fruit pulp extract (JFE) 0.2ml/12h for next 5days(oral gavage). At 16^th days, animals were sacrificed and testes were processed for micrometrical and histopathological study. 

Results: Cr exposure significantly (p≤ 0.001)reduced testicular weight, produce necrotic changes in seminiferous tubules (ST) along with vacuolization. The CSA of ST was increased while Spermatogonia and spermatozoa were decreased. There were apoptic and exfoliated cells while some ST showed desquamation and multinucleated giant cells. The parrot beak headed (PBH) spermatozoa werecommon in testicular smear of Cr group.

Conclusions: Histopathological examination of the specimens showed Cr compounds are highly toxic, while JFE ameliorate toxicities by inhibiting oxidative stress and metal chelating ability.