Effect of Uric Acid on Vitamin C and E in Induced Hyperuricemic Model

Abstract

Objective: To measure the level of uric acids and find out the effect of uric acid on vit C and E  in induced hyperuricemic model.

Study Design: comparative study

Place and Duration of Study: This study was conducted at Baqai Medical University, Karachi from June 2010 to January 2011. 

Materials and Methods: Forty male albino rats with an average weight of 180±2g were selected. The rats were grouped. The animals were fed on stardards diet and given tap water ad libitum until treatment. The  protrocols for experiment was according to institute of laboratory animal resources on life sciences,US National research council,1996 and institutional animal ethical committee(IAEC) of baqai medical university, Karachi. Albino rats were divided into four groups. Group A(10) – control given only standard diet, group B(10) fed on 60%fructose with standard diet , group C(10) fed on fructose, standard diet and intraperitonially oxonic acid 250mg/kg and group D (10) only on injection intraperotonially oxonic acid 250mg/kg. At the end of study 10 ml of blood was drawn from heart of rats. Then blood was estimated for vitamin C, E and uric acids done by kit methods randox-manual /Rx monza UA230/UA 233.

Results: The concentration of vitamin C and E were significantly lowered as compared to uric acid concentration in the group B,C and D.

Conclusion: Decrease level of vit C and E increase the level uric acids were observed. Therefore, it may be suggested that increase intake of vitamin C may be helpful in lowering uric acid concentration.