Anti-Oxidant Status Following Treatment with Ciprofloxacin Toxic Effects on Chondrogenic Cells in Immature Rat Liters

Abstract

Objective: To investigate that in reverse, supplementation of zinc chloride if given simultaneously, can diminish the typical ciprofloxacin-induced chondrotoxicity in immature rat liters.

Study Design: A prospective experimental animal study

Place and Duration of Study: This study was conducted at the Department of Anatomy. Pir Abdul Qadir Shah Jeelani Institute of Medical Sciences Gambat District Khairpur Mir`S from Jan 2014 Dec 2014.

Materials and Methods: Ciprofloxacin & ZnCl2 was administrated to immature rat liters. Ciprofloxacin with a dose of 20 mg/kg body weight & ZnCl2  120 µg/100 gm body weight two times therapeutic dose for 20 days. (From day -1 to day 20 after birth.) Their mean chondrocyte count, chondrocyte size and their nucleus size per field was measured and were compared with similar value of control animals. The results were statistically analyzed to find out the significance.

Results: It was concluded after experimentation that ciprofloxacin administered in immature rat liters decreased mean chondrocyte count, chondrocyte size and their nucleus size per field, decreased by 213.7 ± 0.41, 11.12 ± 0.06 µm and  4.37 ± 0.12 µm  respectively. That ciprofloxacin & ZnCl2 administration maintained the mean chondrocyte count, chondrocyte size and their nucleus size per field maintained by 274.4 ± 0.47, 10.47 ± 0.04 µm and   5.36 ± 0.03 µm respectively. That ZnCl2 administration increased the mean chondrocyte count, chondrocyte size and their nucleus size per field  by  316.0 ± 0.40 , 10.82 ± 0.10 5 µm and 5.69 ± 0.04 µm  respectively.

Conclusion: Application of ciprofloxacin and ZnCl2 post-natal in immature rat liters affected the mean chondrocyte count, chondrocyte size, and their nucleus size per field.  ZnCl2 maintained mean chondrocyte count, chondrocyte size and their nucleus size per field leading to growth in immature rat liters.