Alcohol-Induced Hepatotoxicity: Evaluation of Protective Effects of Vitamin C and Naproxen Based on Hematological and Histopathological Parameters in Rats

Abstract

Objective: To evaluate the protective effect of Vitamin C and Naproxen in Alcohol-induced Hepatotoxicity based on Hematological and Histological Parameters.

Study Design: Experimental Interventional Study

Place and Duration of Study: This study was conducted at the Anatomy Department of Al-Tibri Medical College and Hospital, Isra University Karachi from November 2018 to April 2019.

Materials and Methods: 60 albino rats were selected with an equal gender ratio and placed in three groups. Group A (Control Group) was given purified Ethanol for ten days, Group B (Prophylactic Group) was given Vitamin C and Naproxen prophylactically for seven days after which they were intoxicated with Ethanol for ten days, and Group C was simultaneously given Vitamin C, Naproxen, and Ethanol for ten days. Hematological and Histological parameters were then recorded, whereas data was analyzed using SPSS Version 24.0, and P-Value of ≤0.05 was considered statistically significant.

Results: Group A experienced a severe rise in hematological biomarkers, and a grossly damaged hepatic architectural change was also evidently observed histologically. Group B had also elevated hematological markers above the normal range but lower than Group A. Furthermore, they also had milder cellular damage compared to Group A. Group C produced excellent results by showing within standard range biomarkers and a remarkably near-normal hepatic architecture.

Conclusion: Hepatoprotective effects were observed in the rats' liver due to the antioxidant and anti-inflammatory effects of Vitamin C and Naproxen.