Relationship of Oxyresveratrol on Expression Levels of Tumor Necrosis Factor Alpha and Interferon Gamma in Isoniazid Induced Hepatotoxicity in Experimental Model in Mice

Abstract

Objective: To determine the protective effect against isoniazid induced hepatic-toxicity on expression levels of TNF-α and IFN-γ in immunomodulatory activity of oxyresveratrol.

Study Design: Experimental study

Place and Duration of Study: This study was conducted at the Department of Pharmacology, University of Health Sciences, Lahore, Pakistan from 1^st July 2019 to 31^st December 2019.

Materials and Methods: Mice were given a number from 1 to 35 and were assigned to five groups using lottery method. Each mouse was allotted a number that was written on a piece of small paper. A blinded person picked papers and a mouse whose number was written on paper was assigned group.

Results: The mRNA expression levels of TNF-α were significantly high in INH group as compared to control group (795.9 ± 47.45 vs 417.4 ± 6.55). Oxyresveratrol treatment significantly downregulated expression levels of TNF-α as compared to INH group (372.3 ± 10.27 vs 795 ± 47.45). Oxyresveratrol treatment significantly reduced expression levels of IFN-γ as compared to INH group (225.7 ± 15.57 vs 798 ± 36.9).  Combination therapy showed a significant decreased in expression levels of IFN-γ as compared to INH group (117.7 ± 33.89 vs 798 ± 36.9). Oxyresveratrol showed a significant reduction in interferon γ expression levels as compared to silymarin (225.5 ± 15.57 vs 368.9 ± 22.80).

Conclusion: Oxyresveratrol possesses a protective effect against the isoniazid induced hepatic-toxicity. The decrease in expression levels of TNF-α and IFN-γ may explain immunomodulatory activity of oxyresveratrol.