Effect of Nigella Sativa on Platelets in Organophosphorus Induced Toxicity in Albino Rats

Abstract

Objective: To evaluate the effect of Nigella Sativa on Platelets functions in Organophosphate induced toxicity
in Rats.

Study Design: Experimental Study

Place and Duration of Study: This study was conducted at the Al Tibri Medical College Isra University Karachi Campus from January 2015 to July 2015.

Materials and Methods: Thirty albino rats were selected through random sampling and divided into three groups. Group A was the control group, Group B the group that was induced with Chlorpyrifos, whereas Group C was the interventional group in which the Chlorpyrifos and Nigella Sativa was given. At the end of the experiment, the rats were sacrificed and blood samples were collected via cardiac puncture, after which Platelet count was assessed. Data was analyzed using SPSS. To compare the mean values one-way ANOVA, followed by Post Hoc Tukey’s test
was done.

Results: The mean platelet count of the groups were 185x10³/m³ for Group A, 95x10³/m³ for Group B, and 115x10³/m³ for Group C. Significant difference was seen in the mean platelets count between Group A and B (P-value ≤0.01), and Group B and C (P-value ≤0.01). No significant difference was seen between Group A and C.

Conclusion: Nigella Sativa had a protective effect on Platelet count and hence function when Albino rats were subjected to Organophosphorus Poisoning.