To Evaluate the Neurotropic Effects of Methylcobalamin on Atrophied Cerebellar Granular Cell Layer in Albino Rats Tazeen Kohari1, Faryal Azhar2, Meshaal Azhar2 and Usama Faruqui3

Abstract

Objective: As there is scanty research literature on the neurotropic effects of Methylcobalamin on the Cerebellar Granular cell layer of cerebellum, our present study was carried out to evaluate the neurogenetic effects of methylcobalamin on Cerebellar granular cell layer.

Study Design: Observational, Experimental study

Place and Duration of Study: This study was conducted at the Animal House of Basic Medical Sciences Institute (BMSI) JPMC, Karachi from 30 June and ended at 30 July 2013.

Materials and Methods: We selected 18 albino rats weighing 140-185 animals and each group contained six animals. In Group A there were six, animals which were fed Lab diet and water, Group B contained six albinos which were given Lithium carbonate at a dose of 20 mg/g OD for four weeks  and Group C  received Lithium carbonate at a dose of 20 mg/kg OD  in flour pellets along with Injection  500 microgram of Methylcobalamin.

Results: The results showed regeneration and improved thickness of Granular layer due to vitamin b12.

Conclusion: The strength of this study proved that the use of vitamin b12 is resulted in restoring the cerebellar Granular cell layer of cerebellar gray matter.