Role of Amaltas and Dandasa in Controlling Biofilm Formation of Streptococcus Mutans and Pseudomonas

Abstract

Background: People usually give least importance to their oral hyiegene as a consequence they are suffering from so many disease due to oral biofilm that form over the teeth. Also when patient is going for dental procedures they were exposed to  water from the dental lines of this unit. Pseudomonas aeroginosa were the main culprit present in these water line and form biofilm there as well as in host causing infection .Biofilm forming property confers virulence characteristics thus making such bacteria highly resistant to commonly used antibiotics. 

Objective: We analyzed the antibiofilm activity of natural occurring substances like dandasa (juglans regia) and amaltas( Cassia fistula).

Study Design: Experimental study

Place and Duration of Study:  All clinical isolates were identified at BUMDC and PNS microbiology lab(Karachi) by standard biochemical methods. This Study was conducted between  Nov 2012 to Feb 2013

Material & Methods: 40 streptococcus mutan and 32 pseudomonas aerogenosa samples were taken from oral biofilm and contaminated denal lines water respectively and characterized using conventional biochemical, cultural and molecular methods.Biofilm forming activity of these isolates were checked. Then I mixed the dandasa and amaltas  and observe the antibiofilm forming activity. 

Results: Both dandasa and amaltas in a conc. of 12.5mg/ml and 3.2mg/ml respectively show good antibiofilm forming activity against pseudomonas aerogenosa and in a conc 0.8mg/ml and 12.5 mg/ml respectively against streptococcus mutan.But combinations of dandasa with amaltas were found to be not more effective in inhibiting biofilm formation suggesting a indifferent activity with antiadhesive index of 1.0 and 0.75 against Streptococcus mutans,and pseudomonas aerogenosa  respectively.

Conclusion: Streptococcus mutans in oral biofilm and pseudomonas in dental water lines exhibited biofilm formation which is the cause for antibiotic resistance and providing shelter to other organism .Amaltas  and Dandasa provide a good antibiofilm activity individually .