Histological Study of Preventive Role of Cyanacobalamin (Vitamin B-12) On Heat Induced Testicular Injury

Abstract

Objective: The purpose of this study was to investigate the possible role of Cyanacobalamin (Vitamin B-12) in reducing the hazardous effects of heat on seminiferous tubules of testes in albino rats.

Study Design: Experimental Study

Place and Duration of Study: This study was conducted in the Department of Anatomy, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre, Karachi, for 6 weeks from October 2010 to November 2010.

Materials and Methods: Thirty adult albino rats of 200-250 grams of weight and 90-120 days of age were taken for this study. They were divided into three groups A (control), B (heat treated), and C (heat plus Cyanocobalamin treated). They were further subdivided into A1&A2, B1&B2 and C1&C2, based on duration of treatment of 4 weeks and 6 weeks respectfully. At the end of study histological examination of seminiferous tubules of testes were seen by applying Periodic Acid Schiff Iron Hematoxylin stain.

Results: There was marked damaging effects of heat (42⁰C) on seminiferous tubules of testes with disorganized germinal epithelium and vacuolation. This damage to spermatogenic cell series was well protected with concomitant treatment with Cyanacobalamin (vitamin B-12). There was restoration of germinal epithelium and marked decrease in vacuolation.

Conclusion: This study proved protective role of Cyanacobalmin (Vitamin B-12) in heat induced damage in testes of albino rats.