Protective Effect of Vitamin E (Α Tocopherol Acetate) on Gentamicin-induced Nephrotoxicity in Adult Albino Rats

Abstract

Objective: To determine the preventive role of Vitamin E on renal parenchyma after given  of  gentamicin in adult albino rats.

Study Design: Experimental study.

Place and Duration of Study: This study was carried out in the Department of Anatomy Baqai Medical University and Muhammad Medical College, Mirpurkhas from June 2011 to November 2011.

Materials and Methods: For this study, 30 adult albino rats were taken. They were divided into three groups ; A, B and C. The animals in group-A given normal saline 10 ml/kg/day intraperitoneal for 2 weeks. Group-B received gentamicin 100 mg/kg/day intraperitoneal for 2 weeks and group-C receives gentamicin 100mg/kg/day intraperitoneal with vitamin-E 2 mg/kg/day orally for 2 weeks. On day 15 all animals were sacrificed with deep ether anesthesia. Their kidneys were removed, fixed in 10 % formalin. Representative blocks were taken and embedded in liquid paraffin. For routine histological examination 5 µm thick section cut by microtome and stained with H&E, PAS and silver methenamine. Renal histology was done under light microscope to see the proximal and distal tubular diameter and count

Results: No significant (P>0.05) changes were observed in the histopathology of kidney tissues of the groups A and C rats. The group B significantly (P<0.001) affected the histopathology of kidney.

Conclusion: It may be concluded that gentamicin produces changes in kidney, which may be attributed to ischaemia resulting in tubular necrosis in adult albino rats simultaneous administration of vitamin-E partially protect the morphological and histological changes induced by gentamicin.