Detection of Escherichia Coli Strains among Bacteriuria of Diabetic and Non-Diabetic patients, With Their Antibiotic Resistant and Hematuria Containing Enterotoxin (Sea) Gene

Abstract

Objective: Detection of Escherichia coli Strains among bacteriuria of diabetic and non-diabetic patients

Study Design: Descriptive (cross-sectional) study
Place and Duration of Study: This study was conducted at the Department of Biology, College of Science, University of Basrah, Basrah, Iraq from 2nd March 2024 to 3rd April 2025.

Methods: Out of 42 Gram-positive and Gram-negative bacterial isolates from diabetic and non-diabetic patients, across 26 antibiotics, 89 pattern results were observed versus 83. Three Escherichia coli isolates that shared identical biochemical profiles and antibiotic resistance patterns were found to be genetically different strains using random amplified polymorphic DNA – polymerase chain reaction.

Results: The 22 bacteria isolated from diabetic patients showed that 17 (77%) had the Seagene, with 17 (100%) hematuria samples, compared to 13 (65%) with the Sea gene, with 8 (61%) hematuria among 20 bacteriuria samples from non-diabetic patients.

Conclusion: The bacteriuria from diabetic patients exhibited high resistance for Ticarcillin, Piperacillin and Ciprofloxacin antibiotics revealing a significant difference from that bacteriuria of non-diabetics’ patients. There is a relationship between the presence of enterotoxin and hematuria.