Study of SLC25A12 Gene in Pediatric Age Group with Autism Spectrum Disorder Aged 3-13 Years in Thi-Qar Center of Autism

Abstract

Objective: To find out the mutation in gene SLC25A12, rs2292813 (T>C) single nucleotide polymorphic is a cause of autism spectrum disorder.

Study Design: Experimental study

Place and Duration of Study: This study was conducted at the Thi-Qar Autistic Center in Nasiriya city in Thi-Qar Governorate from 15^st November 2022 to 15^th of June 2023.

Methods: Ninety six patients were diagnosed as cases of autism spectrum disorder by pediatric psychiatry were enrolled and age range was 3 to 13 years. The apparent healthy control group is consisting 96 of children with ages range was 3-13 years. The polymerase chain reaction-tetra-ARMS method was conducted using a commercially available polymerase chain reaction master mix  according to the instructions and special primers were designed to detect rs2292813 (T>C) single nucleotide polymorphic mutation.

Results: The heterozygous genotype (CT) is observed in 10 (10.4%) of samples and homozygous genotype (CC) is observed in 84 (87.5%) of samples while homozygous genotype (TT) is observed in 2 (2.08%) of samples. For control group the heterozygous genotype (CT) is observed in of samples 26 (27.1%) and homozygous genotype (CC) is observed in 69 (71.8%) of samples while homozygous genotype (TT) is observed in 1 (1.04%)

Conclusion: There is statistical association between SLC25A12 single nucleotide polymorphism and autism spectrum disorders